end of testing Tuesday.
Following Sunday's Valencia MotoGP season finale, an official test session is being held at the circuit from Monday to Wednesday.
The test marks the first appearance for Cal Crutchlow at Ducati,
Nicky Hayden at Aspar, Moto2 world champion Pol Espargaro at Tech 3 and former title rival Scott Redding at Gresini.
Redding, Hayden and Hiroshi Aoyama are riding the new Open class Honda Production Racer, with Hayden - who sat out Monday due to limited machine numbers - getting his first ride on the RCV1000R today.
The new privateer Forward Racing Yamaha made its public debut on Tuesday in the hands of Aleix Espargaro and Colin Edwards, when new world champion Marc Marquez and Repsol Honda team-mate
Dani Pedrosa were also back on track after skipping day one.
Marquez led for almost the entire day, lapping under his new official pole record. The 20-year-old - riding with a special helmet design - saved the 2014 RC213V with his knee and elbow after a big lowside moment at turn six, shortly after 1pm.
Like many riders, Marquez made a last push - clocking his best time of the day on the 75th of 76 laps. Yamaha's title runner-up
Jorge Lorenzo moved into second, while Pedrosa reclaimed third from LCR's Stefan Bradl.
Valentino Rossi was working with his new crew chief Silvano Galbusera for the first time, while former Aprilia Racing boss Gigi Dall'Igna is present in his new role as general manager of Ducati Corse.
Former Aspar rider
Randy de Puniet made his PBM debut on Tuesday, as the British team seeks to reach an agreement with Aprilia for 2014. The test was briefly red flagged due to a late fall for PBM regular Michael Laverty.
The 2014 rules will see the Factory bikes lose a further litre of race fuel relative to the Open machines, raising the difference to four litres. The Open bikes must run the full control ECU system, while the Factory entries can use their own software.
The final day of testing begins at 10am on Wednesday, but some teams - including Yamaha - are expected to pack up this evening.
CLICK HERE for more pictures from the test...
1.
Marc Marquez ESP Repsol Honda Team (RC213V)
1m 30.536s (Lap 75/77)
2.
Jorge Lorenzo ESP Yamaha Factory Racing (YZR-M1)
1m 30.768s (58/60)
3.
Dani Pedrosa ESP Repsol Honda Team (RC213V)
1m 30.948s (67/72)
4.
Stefan Bradl GER LCR Honda MotoGP (RC213V)
1m 30.990s (57/60)
5.
Alvaro Bautista ESP Go&Fun Honda Gresini (RC213V)
1m 31.208s (41/72)
6.
Bradley Smith GBR Monster Yamaha Tech 3 (YZR-M1)
1m 31.397s (77/78)
7.
Valentino Rossi ITA Yamaha Factory Racing (YZR-M1)
1m 31.414s (56/57)
8.
Andrea Dovizioso ITA Ducati Team (GP13)
1m 31.661s (55/62)
9.
Pol Espargaro ESP Monster Yamaha Tech 3 (YZR-M1)
1m 31.836s (66/72)
10.
Andrea Iannone ITA Energy T.I. Pramac Racing (GP13)
1m 31.844s (61/68)
11.
Cal Crutchlow GBR Ducati Team (GP13)
1m 32.114s (53/53)
12.
Michele Pirro ITA Ducati Test Team (GP13)
1m 32.473s (47/65)
13.
Nicky Hayden USA Power Electronics Aspar (RCV1000R)
1m 32.576s (76/76)
14.
Yonny Hernandez COL Ignite Pramac Racing (GP13)
1m 32.800s (26/27)
15.
Aleix Espargaro ESP NGM Forward Racing (FTR-Yamaha M1)
1m 32.847s (17/18)
16.
Colin Edwards USA NGM Forward Racing (FTR-Yamaha M1)
1m 33.149s (21/34)
17.
Michael Laverty GBR Paul Bird Motorsport (PBM-ART)
1m 33.672s (32/43)
18.
Randy de Puniet FRA Paul Bird Motorsport (ART)
1m 33.833s (22/37)
19.
Scott Redding GBR Go&Fun Honda Gresini (RCV1000R)
1m 34.541s (22/23)
20.
Mike di Meglio FRA Avintia Racing (FTR-Kawasaki)
1m 34.618s (29/42)